Our lab seeks to dissect the interplay between redox processes and age-dependent changes in tissue function in the nematode C. elegans, in order to shed light on the association between the dysregulation of the cellular redox environment and many human diseases of aging.
Our research focuses on redox processes that span increasing levels of organismic structure—from the molecular to the cellular and organismic levels. We study how oxidation affects the function of proteins and signaling pathways at the molecular level, using molecular biology and CRISPR-Cas9 genome editing. We probe how protein oxidation is regulated at the cellular and tissue levels, using genetically-encoded protein oxidation sensors that we monitor via ratiometric fluorescence microscopy in live animals. We use system-biological tools to integrate how redox control affects organismic traits, including lifespan.
We are recruiting curious, enthusiastic and hard working budding scientists to join our team!
We are looking for candidates interested in combining experimental and theoretical approaches to study the regulation of protein oxidation at molecular and cellular levels. Please send Javier your CV and a cover letter describing your research experience and future goals.
Undergraduates interested in using molecular-genetic approaches to study the mechanisms that regulate lifespan in the nematode C. elegans should contact Javier to discuss possible projects. Please submit your CV, transcript and a brief paragraph detailing why you want to work in our lab.
PhD in Biochemistry, University of California, San Francisco, 1999
BSc in Biology, MIT, 1994
BS in Biochemistry, University of Washington, Seattle, 2015
BS in Biomedical Sciences, University of Central Florida, 2015
Graduate Rotation Student
BA in Biology, Boston University, 2016
Research Assistant in Robert D'Amato's lab, Boston Childrens Hosptial
Summer Student 2016
Medical Student at Seoul National University
Graduate Rotation Student 2016
Graduate student in Erin Cram's lab, Northeastern University
An insulin-to-insulin regulatory network orchestrates phenotypic specificity in development and physiology
Fernandes de Abreu D, Caballero A, Fardel P, Stroustrup N, Chen Z, Lee K, Keyes WD, Nash ZM, López-Moyado IF, Vaggi F, Cornils A, Regenass M, Neagu A, Ostojic I, Liu C, Cho Y, Sifoglu D, Fontana W, Lu H, Csikasz-Nagy A, Murphy C, Antebi A, Blanc E, Apfeld J*, Zhang Y, Alcedo J, Ch'ng Q. PLoS Genetics (2014).
*Co-corresponding and co-last author.
Hulme SE, Shevkoplyas SS, McGuigan AP, Apfeld J, Fontana W, Whitesides GM. Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of elegans. Lab on a Chip (2010).
Alper S, McElwee MK, Apfeld J, Lackford B, Freedman JH, Schwartz DA. The Caenorhabditis elegans germ line regulates distinct signaling pathways to control lifespan and innate immunity. J Biol Chem (2010).
Hulme SE, Shevkoplyas SS, Apfeld J, Fontana W, Whitesides GM. A microfabricated array of clamps for immobilizing and imaging elegans. Lab on a Chip (2007).
Bordone L, Motta MC, Picard F, Robinson A, Jhala US, Apfeld J, McDonagh T, Lemieux M, McBurney M, Szilvasi A, Easlon EJ, Lin SJ, Guarente L. Sirt1 regulates insulin secretion by repressing UCP2 in pancreatic beta cells. PLoS Biology (2006).
Apfeld J*, O’Connor G, McDonagh T, DiStefano PS, Curtis R. The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in elegans. Genes and Development (2004). * Corresponding author.
Francis R, McGrath G, Zhang J, Ruddy DA, Sym M, Apfeld J, Nicoll M, Maxwell M, Hai B, Ellis MC, Parks AL, Xu W, Li J, Gurney M, Myers RL, Himes CS, Hiebsch R, Ruble C, Nye JS, Curtis D. aph-1 and pen-2 are required for Notch pathway signaling, gamma-secretase cleavage of betaAPP, and presenilin protein accumulation. Developmental Cell (2002).
Arantes-Oliveira N, Apfeld J, Dillin A, Kenyon C. Regulation of Lifespan by germ-line stem-cells in Caenorhabditis elegans. Science (2002).
Apfeld J, Kenyon C. Regulation of lifespan by sensory perception in Caenorhabditis elegans. Nature (1999). Accompanying News & Views in Nature.
Apfeld J, Kenyon C. Cell nonautonomy of elegans daf-2 function in the regulation of diapause and life span. Cell (1998). Featured on the cover of Cell, with accompanying Minireview.
Finer-Moore JS; Liu L; Birdsall DL; Brem R; Apfeld J; Santi DV; Stroud RM. Contributions of orientation and hydrogen bonding to catalysis in Asn229 mutants of thymidylate synthase. Journal of Molecular Biology (1998).
Curtis D; Apfeld J; Lehmann R. nanos is an evolutionarily conserved organizer of anterior-posterior polarity. Development (1995).